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Abstract. Ca2+ and human cardiac troponin I (cTnI) peptide binding to human cardiac troponin C (cTnC) have been investigated with the use of 2D {1H,15N} HSQC NMR spectroscopy. The spectral intensity, chemical shift, and line-shape changes were analyzed to obtain the dissociation (KD) and off-rate (koff) constants at 30C. The results show that sites III and IV exhibit 100-fold higher Ca2+ affinity...
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