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In the last decade, advances in molecular biology have provided evidence of the genotypic heterogeneity of melanoma. We analysed BRAF,NRAS and c‐KIT alterations in tissue samples from 63 stage III/IV melanoma patients and autologous cell‐lines, using either allele‐specific or quantitative PCR. The expression of BRAF V600E protein was also investigated using an anti‐BRAF antibody in the same tissue...
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