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In this work, we report a novel use of the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of the chromogenic Fe(BPDS)34- (BPDS=bathophenanthroline disulfonic acid) reporter for the visual detection of β-galactosidase (β-Gal) activity and sandwich immunoassay. The enzymatic hydrolysis of the substrate p-aminophenyl-β-D-galactopyranoside can switch on the reduction of Fe3+...
We report a versatile approach for the colorimetric assay of alkaline phosphatase (ALP) activity based on the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of Fe(II)-phenanthroline reporter. In the presence of ALP, the applied substrate ascorbic acid 2-phosphate is enzymatically hydrolyzed to produce ascorbic acid, which then reduces Fe3+ to Fe2+. The complexation of Fe2+...
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