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Etimicin sulfate is a semi-synthetic aminoglycoside which is modified from gentamicin C1a, used as an antibiotic effective both to Gram-positive and Gram-negative bacteria infections. Ten impurities were detected in the bulk drug by LC-ELSD and LC–ESI-MS n . Weakly acidic cation exchange resin, CM-sephadex and silica gel column chromatography were used for the isolation of six impurities....
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