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Biothiols including cysteine (Cys), reduced glutathione (GSH), and hydrogen sulfide (H 2 S) play critical roles in living organisms. New reaction-based fluorescent turn-on probes based on fast thiolysis of NBD (7-nitro-1,2,3-benzoxadiazole) ether were explored for sensing of biothiols in aqueous buffer. The probes showed fast response toward biothiols with more than 100-fold off–on signal...
A FRET-based fluorescence probe was developed for selective detection of H 2 S in aqueous buffer and inside living cells. For this probe, the FRET probe could be cleaved by H 2 S, and the fluorescence of FRET donor is released. The probe is highly selective to H 2 S over other biologically relevant species to give color change for naked eye observation. Confocal imaging indicated...
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