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As an emerging noninvasive blood biomarker, circulating free DNA (cfDNA) can be utilized to assess diagnosis, progression and evaluate prognosis of cancer. However, cfDNAs are not “naked”, they can be part of complexes, or are bound to the surface of the cells via proteins, which make the detection more challenging. Here, a simple method for the detection of Ubiquitin-like with PHD and ring finger...
In this work, a novel dendritic DNA–porphyrin superstructure was designed as mimetic enzyme for the amplified fluorescent detection of DNA. The dendritic DNA superstructure was in situ assembled with three auxiliary DNAs via hybridization chain reaction. With groove interaction between iron porphyrin (FeTMPyP) and double-stranded DNA, the dendritic DNA superstructure is capable to gather abundant...
The design of a label-free aptamer for separation of recognition sequence from signal reporter is significant to ensure the high-efficiency affinity between aptamer and target. This work develops a label-free triple-helix aptamer (THA) as sensing platform for “signal-on” fluorescent detection of thrombin. THA was composed of aptamer sequence and help DNA 1 (H1), which contained the complementary sequence...
This work observed the anodic electrochemiluminescence (ECL) of C 3 N 4 nanosheets (CNNS) for the first time. The ECL emission was 40 times stronger than that from bulk g-C 3 N 4 in the presence of triethylamine (Et 3 N) as a coreactant due to large surface-to-volume ratio, which enhanced the sensitivity for biosensing. At pH 7.0, the CNNS modified electrode...
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