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Effects of pulsed electric sfields (PEF) on the structure of soybean protein isolates (SPI) were studied by Raman spectra (RS). The secondary structure of SPI mainly consisted of α-helix and β-sheet. The changes of Raman characteristic peaks of amide I and amide III indicated that PEF induced the content of β-sheet and random coil to increase. The decrease of peak 921cm-1 showed the lower of α-helix...
The inactivation of soybean lipoxygenase by pulsed electric fields (PEF) was studied. Effects of PEF parameters (treatment time, pulse strength, pulse frequency and pulse width) were evaluated. Soymilk was exposed to pulsed strengths from 20 to 42kV/cm for up to 1036μs treatment time in square wave pulse of bipolar mode. Moreover, pulse frequency (100–600Hz) and pulse width (1–5μs) was also tested...
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