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SCaMC is an ATP‐Mg/Pi carrier protein located at the mitochondrial inner membrane. SCaMC has an unusual N‐terminal Ca2+‐binding domain (NTD) in addition to its characteristic six‐helix transmembrane bundle. The NTD of human SCaMC1 (residues 1–193) was expressed and purified in order to study its role in Ca2+‐regulated ATP‐Mg/Pi transport mediated by its transmembrane domain. While Ca2+‐bound NTD could...
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