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The functional properties of wild type α-tropomyosin expressed in E. coli with an alanine-serine N-terminal leader (AS-α-Tm) were compared with those of AS-α-Tm with either of two missense mutations (Asp175Asn and Glu180Gly) shown to cause familial hypertrophic cardiomyopathy (FHC). Wild type AS-α-Tm and AS-α-Tm(Asp175Asn) binding to actin was indistinguishable from rabbit skeletal muscle ab-tropomyosin...
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