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We investigated the alterations of heparan sulfate proteoglycans produced by vascular endothelial cells after exposure to lead. Bovine aortic endothelial cells were cultured and metabolically labeled with [ 3 H]glucosamine and [ 35 S]sulfate in the presence of lead chloride at 10 μM. Radiolabeled HSPGs were separated by ion-exchange chromatography and either their hydrodynamic...
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