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In this work, we have implemented a new microfluidic chip which can capture and isolate single-cells in a microchamber array for various single-cell assays. Proposed microfluidic chip consists of two PDMS (Polydimethylsiloxane) layers: a microfluidic channel layer for injecting cells and reagents and an air channel for pneumatic actuation. Each microfluidic chip has 27 microchambers for single-cell...
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