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Click DNA ligation promises an alternative to the current enzymatic approaches for DNA assembly, with the ultimate goal of using efficient chemical reactions for the total chemical synthesis and assembly of genes and genomes. Such an approach would enable the incorporation of various chemically modified bases throughout long stretches of DNA, a feat not possible with current polymerase‐based methods...
Nichtnatürlich vernetzte DNA die für das fluoreszierende Protein mCherry kodiert, funktioniert in Humanzellen, wie A. Tavassoli und Mitarbeiter in ihrer Zuschrift auf S. 2394 ff. erläutern. Das Titelbild zeigt humane MCF‐7‐Zellen mit dem durch eine Klickreaktion verknüpften mCherry‐Gen. Die Zellen zeigen die rote Fluoreszenz des exprimierten mCherry‐Proteins, was die korrekte Transkription durch die...
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