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The ability to quantify the local electrostatic environment of proteins and protein/peptide assemblies is key to gaining a microscopic understanding of many biological interactions and processes. Herein, we show that the ester carbonyl stretching vibration of two non‐natural amino acids, L‐aspartic acid 4‐methyl ester and L‐glutamic acid 5‐methyl ester, is a convenient and sensitive probe in this...
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