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Ubiquitination plays an important role in posttranscriptional modification of proteins. As a component of the ubiquitin conjugation system, E3 ligase is believed to be a key element determining the substrate specificity of ubiquitination. In this research, a novel putative U-box type E3 ligase VaPUB (plant U-box protein) was isolated from a very cold-hardy “Zuoshanyi” (Vitis amurensis Rupr. cv.) grapevine...
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