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The quality of nucleic acid solution structures can be significantly improved using residual dipolar coupling data. However, many of the one-bond couplings that could be used for this purpose are difficult to measure. Conventional 2D experiments are often unable to reveal one-bond H2′-C2′ and H3′-C3′ couplings in large RNA molecules due to spectral overlap. Here we show how to use 3D HCcH-COSY and...
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