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In this study, we reinvestigated the role of Afr1p in the regulation of pheromone signaling and demonstrated that pheromone signaling was not regulated by Afr1p because neither deletion nor overexpression of AFR1 affected α‐factor‐induced transcription induction of the FUS1 gene. The enhanced α‐factor resistance resulting from overexpression of AFR1 was dependent on the Slt2p mitogen‐activated protein...
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