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Dip‐pen nanolithography (DPN) is a unique nanofabrication tool that can directly write a variety of molecular patterns on a surface with high resolution and excellent registration. Over the past 20 years, DPN has experienced a tremendous evolution in terms of applicable inks, a remarkable improvement in fabrication throughput, and the development of various derivative technologies. Among these developments,...
Microfluidic encapsulated allergen arrays are created by M. Hirtz and co‐workers on page 5330 using polymer pen lithography (PPL). These arrays are utilized in mast cell activation studies on a single cell level. The overall setup enables easy handling of cell suspension, incubation, and read‐out by fluorescence microscopy, allowing immune cell activation screening to be easily adapted for diagnostics...
The profiling of allergic responses is a powerful tool in biomedical research and in judging therapeutic outcome in patients suffering from allergy. Novel insights into the signaling cascades and easier readouts can be achieved by shifting activation studies of bulk immune cells to the single cell level on patterned surfaces. The functionality of dinitrophenol (DNP) as a hapten in the induction of...
This chapter focuses on surface‐bound lipid assemblies generated by two techniques, dip‐pen nanolithography (DPN) and polymer pen lithography (PPL). In recent years, these two methods (and related stamping techniques based on DPN setups) have emerged into sophisticated platforms for the generation of functional lipid assemblies and supported lipid membranes. PPL emerged as a combination of DPN and...
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