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The feasibility of simultaneous production of acetate and methane from glycerol was investigated by selective enrichment of hydrogenotrophic methanogens in an extreme-thermophilic (70°C) fermentation. Fed-batch experiments showed acetate was produced at the concentration up to 13.0g/L. A stable operation of the continuous stirred tank reactor (CSTR) was reached within 100days. Acetate accounted for...
Hydrogen supersaturation in extreme-thermophilic (70°C) mixed culture fermentation (MCF) was demonstrated for the first time by membrane inlet mass spectrometry. It was found that hydrogen supersaturation ratio (R H2 ) increased dramatically (from 1.0 to 20.6) when H 2 partial pressure (P H2 ) was reduced by N 2 flushing or sparging. The distribution change of metabolites...
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