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A method has been developed to obtain virus-free sweet pepper plants from apical meristems by in vitro grafting. The shoot tips (0.4-0.8mm) excised from virus-infected plants in a green house were grafted onto decapitated rootstock seedlings. The shoot tip was an apical meristem with two to four leaf primordia. Shoot development was observed in 2-83% of grafted shoot tips. By this method, virus-free...
A simple and reliable method for evaluating the viability of Brassica pollen was established in which the in vitro germination rate of pollen was adopted as the index of the viability of pollen grains. Pollen grains were preincubated in an atmosphere in which the relative humidity (RH) was fixed to 52% or 66% at 20 °C for 5 hours. They were cultured for 16 hours at 25 °C in a liquid Kwack's medium...
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