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Sonoporation is the ultrasound induced transient opening of cell membranes. Large scale oscillation of nearby microbubbles (MBs) is considered to be the primary effect in sonoporation whereas MB destruction correlates with lower sonoporation efficiency and increased cell damage. The scale of MB oscillation in a cloud can be determined by monitoring subharmonic emission. In this study, SW480 cells...
The success of the transportation of genes or drugs into cells via sonoporation strongly depends on the applied acoustic pressure. But especially in in vivo applications, locally deposited acoustic energy is unknown since anatomical structures like bones and the lung attenuate and reflect sound waves strongly. Therefore, the local acoustic pressure may not lie within the optimal range for sonoporation...
Support vector machines are applied to extract marker genes from various microarray data sets: breast cancer, leukemia and monocyte - macrophage differentiation to ease classification of related pathologies or characterize related gene regulation pathways.
In this study we focus on classification tasks and apply matrix factorization techniques like principal component analysis (PCA), independent component analysis (ICA) and non-negative matrix factorization ( NMF) to a microarray data set. The latter monitors the gene expression levels (GEL) of mononcytes and macrophages during and after differentiation. We show that these tools are able to identify...
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