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A gene encoding the thermostable α-amylase from hyperthermophilic archaeon Thermococcus hydrothermalis was cloned in Escherichia coli. In an effort to achieve the improved expression, four DNA primers (three reverse and one forward) were designed to yield three recombinant variants of α-amylase. The additional aim of the re-cloning was to verify how the changes at the C-teminal end of the recombinant...
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