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Quantitative real-time PCR (qRT-PCR) is the preferred method for accurate analysis of targeted gene expression. The aim of this study was to validate optimal reference genes for qRT-PCR analyses in pear pollen. Ten candidate reference genes were chosen and examined in 36 pear samples. Among these, PbrEF1α, PbrGAPDH and PbrAPT were developed to detect the purity of cDNA. Three qRT-PCR analysis programs,...
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