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We report a novel approach to concurrently improve the tolerance to ionic liquids (ILs) as well as reduce lignin inhibition of Trichoderma reesei cellulase via engineering enzyme charge. Succinylation of the cellulase enzymes led to a nearly twofold enhancement in cellulose conversion in 15% (v/v) 1‐butyl‐3‐methylimidazolium chloride ([BMIM][Cl]). The improvement in activity upon succinylation correlated...
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