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The toxic effect of ammonia on rCHO-GS cell decreased obviously due to the transfection of GS system in serum-free culture. The maximum cell density, 15.6 × 10 5 cells/mL, was obtained in the culture with 1.42 mmol/L ammonia. The growth of rCHO- GS cell was inhibited with an increased ammonia concentration. However, a cell density of 8.9 × 10 5 cells/mL was obtained when the concentration...
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