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Green‐to‐red photoconvertible fluorescent proteins have been found to undergo efficient photoconversion by a new method termed primed conversion that uses dual wave‐length illumination with blue and red/near‐infrared light. By modifying a confocal laser‐scanning microscope (CLSM) such that two laser beams only meet at the focal plane, confined photoconversion at the axial dimension has been achieved...
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