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We explore nitric oxide (NO) effect on K in+ channels in Arabidopsis guard cells. We observed NO inhibited K in+ currents when Ca2+ chelator EGTA (Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid) was not added in the pipette solution; K in+ currents were not sensitive to NO when cytosolic Ca2+ was chelated by EGTA. NO inhibited the Arabidopsis stomatal opening, but when EGTA...
The effects of BaCl2 on slow vacuolar (SV) currents of radish are studied by using the whole-vacuolar patch-clamp recording mode. The Ca2+-dependent SV channel can be activated by cytosolic Ca2+. When 1 mmol/L BaCl2 is added into pipette solution, SV currents are suppressed remarkably. Then adding BaCl2 of different concentrations into the bath solution, SV currents reflect different effects. The...
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