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The double‐recessive genic male‐sterile (ms) line ms5 ms6 has been used to develop cotton (Gossypium hirsutum) hybrids for many years, but its molecular‐genetic basis has remained unclear. Here, we identified the Ms5 and Ms6 loci through map‐based cloning and confirmed their function in male sterility through CRISPR/Cas9 gene editing. Ms5 and Ms6 are highly expressed in stages 7–9 anthers and encode...