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The fluorescence-based integrated chemical and enzyme sensor arrays on which two kinds of 10 μm i.d. sensor spots were arranged alternately on one chip, were developed by using microcontact printing. FITC, ruthenium complex and europium tetracycline was stamped onto a DLC sputtered glass slides for pH, oxygen and hydrogen peroxide sensors, respectively. After printing of oxygen sensor spots, pH sensor...
We performed single-molecule observation of DNA double-strand breaks caused by Co60 γ-ray irradiation and quantitatively evaluated the protective effect of ascorbic acid 2-glucoside (AA-2G). AA-2G is a stable derivative of ascorbic acid. We found that AA-2G induces a significant conformational change on DNA. We also evaluated the protective effect of AA-2G against radiation-induced DNA double-strand...
This paper describes the single cell based detection and collection system based on respiration or metabolic activity. The micro-arrayed chemical sensors, which we have already developed, were used for parallel monitoring of single cell activity. These sensors consist of optical sensor film for pH or oxygen, and micro well array prepared with carbon black doped PDMS. We have succeeded in a parallel...
We investigated the interaction of large DNA with protamine, a small basic protein, using fluorescence microscopy and electron microscopy. In most vertebrate sperm cells, a large DNA is packaged by protamine into a highly compact, biologically inactive form of chromatin. The manner of packaging plays a key role in spermatogenesis. We found that, with increase of protamine concentration, DNA molecules...
This paper reports a parallel monitoring method for single cell metabolic and respiration activity by using a microarrayed pH and oxygen sensor. Optical sensor film was prepared by immobilizing fluorescein isothiocyanate (for pH sensor array) or dichlorotris (1,10-phenanthroline) ruthenium (II) (for oxygen sensor array) onto a diamond like carbon sputtered slide glass. Micro well (10 mum i.d.) array...
To introduce our preliminary experience with indocyanine green (ICG) fluorescence angiography for the assessment of lower leg bypasses.1ml of 0.5% indocyanine green was intravenously injected in 9 patients with PAD who underwent paramalleolar artery bypass using saphenous vein grafts. A newly developed near-infrared camera system (PDE; Hamamatsu Photonics K.K. Hamamatsu, Japan) was used for this study...
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