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An in vitro two-liquid-phase suspension culture system of Alkanna orientalis was established in order to evaluate the ability of cultured cells to produce naphthoquinone derivatives. Initially, callus tissues were prepared from cotyledon explants of A. orientalis on the solidified B5 medium supplemented with 1 mg/L 2,4-dicholorophenoxyacetic acid (2,4-D) and 2 mg/L kinetin (kin). Subsequently, fragile...
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