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S‐adenosyl‐
l‐methionine (SAM) is a high‐value compound widely used in the treatment of various diseases. SAM can be produced through fermentation, but further enhancing the microbial production of SAM requires novel high‐throughput screening methods for rapid detection and screening of mutant libraries. In this work, an SAM‐OFF riboswitch capable of responding to the SAM concentration was obtained...