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Fragments of ACV synthetase ofPenicillium chrysogenumcarrying partial activities of amino acid activation were expressed under thealcApromoter in anacvA-deletion mutant ofAspergillus nidulans.The 210 kDa domain A-β-galactosidase fusion protein was partially cleaved to fragments of 200 and 97 kDa. The domain A fragment and the 312 kDa domain BC construct were identified by peptide specific antibodies...
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