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Neurotoxicity in primary neurons was induced using hypoxia/hypoglycemia (H/H), veratridine (10μM), staurosporine (1μM) or glutamate (100μM), which resulted in 72%, 67%, 75% and 66% neuronal injury, respectively. 3-Aminopyridine-2-carboxaldehyde thiosemicarbazone (PAN-811; 10μM; Panacea Pharmaceuticals, Gaithersburg, MD) pretreatment for 24 h provided maximal neuroprotection of 89%, 42%, 47% and 89%...
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