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The ability to express and purify modified recombinant signalling proteins such that they retain their biological function in a cell-free context has provided a basis for production of molecular biosensors. Here the authors utilise G-protein coupled receptors (GPCRs) and their G-proteins to detect various binding partners in a cell-free environment. Molecular biology approaches were employed to express...
Functionalised gold nanoparticles, capable of specifically binding histidine-tagged proteins, are of interest as their unique optical properties can facilitate the study of protein-protein interactions. Citrate-stabilized gold nanoparticles were functionalised with surface capping agents to specifically bind histidine-tagged proteins, and to test the stability of the nanoparticle solutions under various...
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