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γ-Decalactone, an important flavor compound, can be produced by Yarrowia lipolytica, but the yield is poor because of lactone degradation by enzyme Aox3 (POX3 gene encoded). A yeast strain Yarrowia lipolytica TA1 of high γ-decalactone yield was constructed by integrating copper-resistance gene CRF1 from Yarrowia lipolytica into the locus of POX3 genes. After being cultured in shake-flask at 28°C for...
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