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An oligonucleotide probe has been designed and compounded for detecting the ethambutol (EMB) resistance gene of Mycobacterium tuberculosis. It was placed on a nitrocellulose membrane for use in the reverse dot-blot hybridization assay, with the PCR product labeled with biotin. Analysis was carried out on 82 M. tuberculosis clinical isolates. The results indicate that among the 31 EMB-sensitive strains,...
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