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A simple, reliable and sensitive liquid chromatography tandem mass spectrometry (LC‐MS/MS) protocol was developed and validated for quantification of bisoprolol in human plasma. The sample was pretreated with a simple procedure of protein precipitation and an isotope‐labeled d5‐bisoprolol was used as internal standard. The chromatographic separation was performed on a Capcell Pak C18 MG III column...
A rapid and sensitive liquid chromatography–isotope dilution tandem mass spectrometry method was developed and validated for quantification of itraconazole (ITZ) and its active metabolite hydroxyitraconazole (OH‐ITZ ) in human plasma. The plasma samples were extracted with tert‐butyl methyl ether and two isotope‐labeled internal standards (D5‐itraconazole and D5‐hydroxyitraconazole) were used. The...
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