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Arginine deiminase (ADI) is a therapeutic protein for cancer therapy of arginine-auxotrophic tumors. However, ADI’s application as anticancer drug is hampered by its low activity for arginine under physiological conditions mainly due to its high “KM” (S0.5) values which are often 1 magnitude higher than the arginine concentration in blood (0.10–0.12 mM arginine in human plasma). Previous evolution...
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