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Oestrogens, such as oestrone (E 1 ), 17β-oestradiol (E 2 ), oestriol (E 3 ) and their biologically active metabolites 2-methoxyoestrone (2-MeOE 1 ), 2-hydroxyoestradiol (2-OHE 2 ) 16-ketooestradiol (16-OE 2 ), 16-epioestriol (16-epiE 3 ), as well as testosterone (T) play an important role in physiological and pathological developmental processes...
A first assay based on stable isotope dilution/gas chromatography-mass spectrometry (ID/GC-MS) has been developed for plasma 11-deoxycortisol (Reichstein's compound S), the leading hormonal marker of 11β-hydroxylase deficiency. A suitable internal standard being unavailable, we synthesized dideuterated 11-deoxycortisol according to a newly devised synthetic procedure. 17,21-Dihydroxy-pregna-1,4-diene-3,20-dione...
A first assay based on stable isotope dilution/gas chromatography-mass spectrometry has been developed for plasma 17α-hydroxypregnenolone, the leading marker of 3β-hydroxysteroid dehydrogenase deficiency. Equilibration of plasma with internal standard was followed by solid phase extraction. After clean up using Sephadex LH-20 mini columns, heptafluorobutyrates were prepared as derivatives. Quantification...
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