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Early biochemical studies have demonstrated that lipid accumulation by oleaginous yeasts is linked to the activity of the NAD+-dependent isocitrate dehydrogenase (Idh). However, molecular study of Idh of oleaginous microorganisms remains limited. Here, we present the cloning of a mitochondrial NAD+-specific Idh from Rhodosporidium toruloides (RtIdh), an excellent microbial lipid producer that uses...
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