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Heat shock treatment is the most popular method for transformation of Escherichia coli. We have used 19-nm Fe3O4 nanoparticles for improving heat shock protocol. PGEM-T (3,000 bp) and pCAMBIA (8,428 bp) were used as test plasmids for transformation of competent E. coli cells (strains DH5α and Jm107) obtained from heat shock- and CaCl2-treated bacteria. A combination of heat shock and Fe3O4 nanoparticles...
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