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This paper describes a methodology that realizes uniformly-sized liposome arrays with gentle hydration. The system consists of a dried lipid pattern on a substrate that is a micropatterned polymer thin film on an indium-tin-oxide (ITO) glass slide. An electrospray deposition (ESD) method was applied to obtain the lipid patterning at the polymer pattern where the ITO surface was exposed. With a simple...
We present a cell handling method using magnetically functionalized microplates. Rectangular-shaped permalloy inside the microplates enables the plates to rotate or flip upside down by magnetic field. We conduct two kinds of cell handling experiments: (1) rotating cells for the precise positioning of cell nucleus and (2) flipping over the microplate for culturing different types of cells on both sides...
We describe a method for generating giant (micro sized) lipid vesicles using pneumatic valves and microfluidic T-junction. Water, oil containing lipids, and water were sequentially infused into the microfluidic channel to form bilayer lipid membranes (BLMs) on chambers (Fig. 1). Valves on the back of the chambers were driven pneumatically and pushed the water out of the chamber; consequently the BLMs...
This paper describes a method to transplant neurospheroid network onto the rat brain. We first patterned the uniform sized neurospheroids on the PDMS microchamber, and cultured for 1-2 weeks. After 2-week culture, the neurospheroids tightly connected each other with extending their neuronal processes (e.g. dendrite, axons) and formed neuronal network. We realized that the neurospheroid network can...
This paper describes core-shell hydrogel wires for constructing 3D heterogeneous hydrogel microstructures containing biomaterials. A core hydrogel layer contains biomaterials such as cells, and a shell hydrogel layer covers the core to realize a mechanically-robust hydrogel wire. Using this core-shell gel wires, we demonstrate a woven sheet with heterogeneous gel wires by using our stereolithographically-made...
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