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With the development of the Protein Standard Absolute Quantification (PSAQ method), the use of isotopically labeled analogue standards and MS analysis was transferred from the field of small molecules to the field of proteomics analysis. In this article, Dr Virginie Brun and colleagues focus on the use of PSAQ standards for absolute quantification of biomedically relevant proteins. They start by describing...
Absolute protein quantification, i.e. determining protein concentrations in biological samples, is essential to our understanding of biological and physiopathological phenomena. Protein quantification methods based on the use of antibodies are very effective and widely used. However, over the last ten years, absolute protein quantification by mass spectrometry has attracted considerable interest,...
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