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We have developed a microtiter assay for evaluating basophil spontaneous adhesion to extracellular matrix (ECM) proteins examplified by fibronectin and cytokine induced basophil adhesion to bovine serum albumin (BSA). The percentage of basophils adhering to either ECM or BSA was quantified by the histamine content of the adhering basophils. The spontaneous adhesion to fibronectin was higher than to...
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