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The present study envisages expression of immunodominant ectodomain of peste des petits ruminants virus (PPRV) fusion (F) protein in Escherichia coli BL21 (DE3) and its characterization to assess its immunoreactivity. The ectodomain gene sequences corresponding to 222 amino acids, was amplified from PPR vaccine virus, cloned into pET33b vector and expressed in E. coli at an optimal temperature of...
A highly sensitive and specific reverse transcription polymerase chain reaction enzyme linked immunosorbent assay (RT-PCR-ELISA) was developed for the objective detection of nucleoprotein (N) gene of peste des petits ruminants (PPR) virus from field outbreaks or experimentally infected sheep. Two primers (IndF and Np4) and one probe (Sp3) available or designed for the amplification/probing of the...
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