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High performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) was optimized for the analysis of oligosaccharides derived from the extracellular matrix component hyaluronan. Using this sensitive approach, the separation of oligosaccharides consisting of two (molecular weight ca. 0.8kDa) up to 25–30 (molecular weight: ca. 9.5–11.4kDa) disaccharide moieties was possible...
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