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DNA measurements of few, specific cells are done usually with a fluorescence microscope combined with sensitive camera systems (SIT, MCP, cooled CCDs) and a frame grabber for image analysis /7/. The fluorescent cells could be observed on the monitor of the image analyser. In almost all cases the DNA content of some specific cells is determined interactively. It is desirable to automate this time-consuming...
Die Inzidenz des Mammakarzinoms liegt in den westlichen Ländern bei 70–80/100.000 Frauen/Jahr. Der Häufigkeitsgipfel liegt im 5. und 6. Lebensjahrzehnt. Durchschnittlich werden in Deutschland jährlich etwa 45.000 neue Fälle von Brustkrebs diagnostiziert. Die Überlebensraten über alle Stadien betragen nach 10 Jahren etwa 60%. Das Mammakarzinom verursacht etwa 20% aller Krebstodesfälle der Frau.
The aim of the present study was to screen early and advanced pancreatic cancer (PC) for analogous and different cytogenetic changes and to correlate the detected aberrations with clinical outcome. Paraffin-embedded specimen of 33 patients with PC were investigated for gains and losses of genetic material by comparative genomic hybridisation (CGH) after separation of PC from normal cells by...
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