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In order for a protein to elicit a systemic allergic response it must reach the circulatory system through the intestinal mucosa as a sufficiently large fragment with adequate structural integrity. Sunflower LTP and 2S albumins (SFA8 and three mixed fractions of Alb1 and Alb2) were digested in simulated gastric fluid (SGF) for 2h and the conditions were then changed to mimic the intestinal environment...
An M r 22 500 protein was purified from isolated oil bodies from sunflower cotyledons. N-terminal amino acid sequencing showed that this protein belonged to the 2S albumin group of storage proteins. A corresponding cDNA clone encoded a preproprotein, comprising two mature 2S albumin proteins one of which corresponded to the oil-body associated albumin. In contrast, the second albumin encoded...
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