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To induce antibodies in vitro we used a medium containing enriched RDF supplemented with human plasma, fetal calf serum, IL-2, IL-4, IL-6, and muramyl dipeptide. Human lymphocytes were cultivated at a final density of 1 × 106 cells/ml in the medium of 96- or 24-well plates in a CO2 incubator at 37°C for 10 days. After 10 days, about 100 ng/ml of IgE antibody was secreted into the medium. The technique...
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