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Disulfide bond formation during protein folding of nascent proteins is associated with the generation of H 2 O 2 in the endoplasmic reticulum (ER). Approaches to quantifying H 2 O 2 directly within the ER failed because of the oxidative environment in the ER lumen, and ER-specific catalase expression to detoxify high H 2 O 2 concentrations resulted in...
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