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Nep1 methylates the hypermodified ψ1191 base of 18S rRNA and has an additional essential function during ribosome biogenesis. It is strongly conserved in eukaryotes and a point mutation causes the human Bowen–Conradi syndrome. To identify Δnep1‐specific genetic interactions, viable deletions were screened genome‐wide (SGA). Due to its essential function, we used, for the first time, query strain (...
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